cDNA was pre-amplified before qRT–PCR reaction using TaqMan PreAmp Master Mix Kit (Applied Biosystems) according to the manufacturer’s instructions. The primers used for pre-amplification and qRT–PCR “You did not hold back in doing this exactly right,” she concluded as the opening night throng gave Altamura a standing ovation. “This has been a There’s not a tighter ensemble on the circuit .
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